Tag Archives: Amniotic membrane

Assessment of Antibiotic Influence on Structural Modifications of Amniotic Membrane by FTIR Spectroscopy

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By George Roiu, Simona Cavalu et al.

https://doi.org/10.37358/MP.20.2.5365

The purpose of our study was to investigate the structural modifications of collagen extracellular matrix of amniotic membrane upon interaction with two different antibiotics, frequently used in surgical and post- surgical procedure, respectively ciprofloxacin and gentamicin. SEM micrographs evidenced the ultrastructure features of dried amniotic membrane, with laminar structure, flexible, transparent, with no blood vesels or nerves. FTIR spectroscopy combined with deconvolution techniques was applied with the aim to determine the extent of denaturation upon treatment with different antibiotics. By spectral analysis, we concluded that gentamicin treatment is more favorable compared to ciprofloxacin, as the denaturation process is reflected by the lower sheet/turns ratio of the secondary structure composition.

Photographic image of amniotic membrane; (b) SEM micrograph recorded on the surface of amniotic membrane; (c) SEM cross-section image of amniotic membrane.
Copyright Simona Cavalu et al.
FTIR spectra of amniotic membrane: (a) air dried, natural; (b) after gentamicin treatment; (c) after ciprofloxacin treatment. Copyright Simona Cavalu et al.
Quantitative analysis (percent) based on spectral deconvolution of amide I FTIR absorption band of natural amniotic membrane and threated with gentamicin and ciprofloxacin. Copyright Simona Cavalu et al.

The present study was devoted to structural analyses of amniotic membrane as a potential natural biomaterial for biomedical applications, including tissue regeneration. The basement membrane closely resembles to that of the conjunctiva and cornea, especially with regards to its collagen composition (collagen type IV, V and VI) in addition to fibronectin and laminin. SEM micrographs evidenced the ultrastructure features of dried amniotic membrane, with laminar structure, flexible, transparent, with no blood vessels or nerves. FTIR spectroscopy combined with deconvolution techniques was applied in order to determine the extent of amniotic membrane denaturation upon treatment with different antibiotics. By spectral analysis, we can conclude that the gentamicin treatment is more favorable compared to ciprofloxacin, as the denaturation process is reflected by the lower sheet/turns ratio.(Copyright Simona Cavalu et al.)

Amniotic membrane for ophthalmologic surgery

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By Simona Cavalu et al.

Nano-Scale Modifications of Amniotic Membrane Induced by UV and Antibiotic Treatment: Histological, AFM and FTIR Spectroscopy Evidence

The efficiency of amniotic membrane (AM) transplantation in different types of ocular surface disorders is due to its outstanding properties such as antifibrotic, antibacterial, anti-inflammatory and antiangiogenic, working as a versatile scaffold to promote corneal tissue epithelialization.

Recurrent pterygium excision. Copyright Simona Cavalu et al.
Amniotic membrane used in ophthalmologic surgery to cover the remaining tissue defect due to the grade 3 recurrent pterygium excision: (a) grade 3 recurrent pterygium, preoperative appearance; (b) intraoperative aspects (the same patient); (c) intraoperative preparation of the amniotic membrane fragment to be applied on the remaining defect following recurrent pterygium excision; (d) intraoperative appearance—application of the AM fragment on the remaining defect and 10.0 thread suturing to the bulbar conjunctiva of the fragment; (e) the aspect of the patient’s cornea four weeks after surgery . Copyright Simona Cavalu et al.
Histological and immunohistochemical examination of amniotic membrane. Copyright Simona Cavalu et al.
 Histological and immunohistochemical examination: (a,b) natural amniotic membrane (AMN); (c,d) amniotic membrane exposed to UV for 1 h (AUV); (e,f) amniotic membrane treated with gentamicin (40 mg/mL) (AG40); (g,h) amniotic membrane treated with gentamicin 80 mg/mL (AG0); (i,j) amniotic membrane treated with gentamicin 40 mg/mL and exposed to UV for 1 h (AGUV). Left panel: H&E staining; right panel: immunohistochemistry staining of collagen IV (antibody clone CIV 22). Scale: 100 μm. Copyright Simona Cavalu et al.

The full text of the paper is available at:

https://www.mdpi.com/1996-1944/14/4/863/htm