Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of Stellaria media (L.) Vill

By F. M. Groza, S. I. Vicas, Simona Cavalu et al.

Stages of wound healing and the implications of fibroblasts in this process.
Copyright F. Groza, Simona Cavalu et al.

The main aim of our work was to highlight the in vitro healing potential of Stellaria media (L.) Vill. (SM) extract using the scratch assay on normal human dermal fibroblasts (NHDF). The ability to stimulate cell migration and proliferation under the influence of different concentrations of SM extract (range between 12.5 and 200 µg/mL) was determined compared to the control (untreated in vitro-simulated wound) and positive control (allantoin 50 µg/mL).Our findings demonstrate, for the first time in the literature, the wound-healing potential of SM extract on NHDF using the in vitro scratch method. 

Effect of Stellaria media extract concentrations (between 12.5 µg/mL and 200 µg/mL) on viability of NHDF after 24 h. Copyright F. Miere, Simona Cavalu et al.
The evolution over time (0, 12, 24, 36, and 48 h) of cell wound coverage at different concentrations of Stellaria media (L.) Vill extract (range 200 µg/mL–12.5 µg/mL) compared to positive control (CTRL)—treated with allantoin 50 µg/mL and untreated wound control (CTRL0). Fibroblasts marked in pink are those that have mobilized into the wound. The scale of the processed images is 100 µm. Copyright F. Groza, S. I. Vicas, Simona Cavalu et al.
The evolution of the cell density parameter inside the wound depending on the applied treatment and time. Copyright F. Groza, Simona Cavalu et al.
Evolution over time, of the average values of the widths (µm) of the wound, the areas (mm2) of the wound, of the normalized densities (%) of the cells inside the wound, and the Lp norm, for all treatments. C200—12.5 µg/mL represents the Stellaria media (L.) Vill extract concentrations applied, CTRL—positive control (allantoin—50 µg/mL), CTRL0—control (without treatment), where no treatment was applied. T00–T48 represents the time expressed in hours. Copyright F. Groza, Simona Cavalu et al.

Full text at https://www.mdpi.com/2076-3417/11/23/11526/htm