Perspectives on the Combined Effects of Ocimum basilicum and Trifolium pratense Extracts in Terms of Phytochemical Profile and Pharmacological Effects

By A. I. Antonescu, Simona Cavalu et al

Common and distinct therapeutic effects of Ocimum and Trifolium species.
Copyright I.A. Antonescu, Simona Cavalu et al.
Different species of Trifolium according to the amount of cyanogenic glycosides and isoflavones and identifying the species with rich chemical composition.
Copyright I.A. Antonescu, Simona Cavalu et al.
Pharmacological activities of extracts from Trifolium and Ocimum species after internal and external administration. Copyright I. A. Antonescu, Simona Cavalu et al.
Stages of the wound healing process (adapted from [121]).
Copyright I.A. Antonescu, Simona Cavalu et al.

The ability to promote wound healing by synergic effect of Trifolium Pratense and Ocimum basilicum mixt extract has not been studied yet, being the central point for future studies. So far, the anti-inflammatory, antimicrobial, antifungal and anticancer properties have been demonstrated for each extract individually, obtaining promising results, and for these reasons, in the future, the mixture of both extracts are of great interest to be studied, expecting for a potential synergistic effect.

To the best of our knowledge, there are no studies in the literature dealing with any in vitro tests of the mixture Trifolium pretense and Ocimum basilicum extracts. Our research group performed a preliminary study using the “scratch test” assay on human fibroblasts, by applying the extract mixture in different concentrations on fibroblasts culture, in order to evaluate the optimum concentration to promote the stimulation and proliferation of the cells. Within this test, which is an in vitro model of wound healing, human fibroblasts were primarly grown to a confluent monolayer, and then was scraped in a straight line with a pipette tip, in order to simulate a wound. The fibroblasts migration into the wound area was monitored during 48 h incubation in the presence of different concentrations of mixed plant extracts along with the control (no treatment). Copyright I.A. Antonescu, Simona Cavalu et al.

The spontaneous migration of dermal fibroblasts is evidenced under light microscopy, along with the control samples, showing the progressive covering of the pseudo-wound monitored at different times intervals. Copyright I.A. Antonescu, Simona Cavalu et al.
Wound healing percent express as fibroblast migration to cover the scratched area. Copyright I.A. Antonescu, Simona Cavalu et al.

The antioxidant, antimicrobial, antiviral, antifungal and anti-inflammatory activity of Ocimum and Trifolium species are summarized in this review in order to explore the therapeutic potential of Ocimum basilicum and Trifolium pretense in relation with their phytochemical profile and to highlight the pharmacological activity of aqueous or ethanol extracts. Special attention was devoted to the dermal pathology and wound healing effects, in the context of multiple skin conditions such as acne, eczema boils, psoriasis and rashes. Both extracts (Trifolium sp. and Ocimum sp.) are characterized by high content of antioxidant compounds, which are also responsible for the radiance and resistance of the skin and the slowing down of the aging process by maintaining estrogen levels. Moreover, the potential combined effect of the mixed extract is pointed out in terms of future applications for wound healing, based on some preliminary results obtained from a “scratch tests” assay performed with respect to human dermal fibroblasts. Copyright I.A. Antonescu, Simona Cavalu et al.

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