Tag Archives: photochemistry

Growth, Photosynthetic Pigments, Phenolic, Glucosinolates Content and Antioxidant Capacity of Broccoli Sprouts in Response to Nanoselenium Particles Supply

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By S. I. Vicas, Simona Cavalu et al.

The aim of our study was the biofortification of broccoli sprouts with selenium nanoparticles (NSePs) and evaluation of growth parameters, assimilator pigments content, total phenols, glucosinolates content along with antioxidant capacity, in order to boost value added output, such as improved nutrition and food functionality. Copyright Simona Cavalu et al.

a) Size distribution of NSePs b) 3D micrograph of Selenium nano-spheres .
Copyright Simona Cavalu et al.
Effect of NSePs particles supply on: a) Total biomass; b) Root weight; c) Shoot weight. Copyright Simona Cavalu et al.
Effect of NSePs treatment on green pigments content and total carotenoids.
Inset, chlorophyll a/chlorophyll b ratio. Copyright Simona Cavalu et al.
Effect of NSePs treatments on: a) total phenols content; b) antioxidant capacity determined by DPPH assay; c) antioxidant capacity determined by FRAP assay. Copyright Simona Cavalu et al.
FTIR spectra of broccoli sprouts leaves upon NSePs treatment: a) 100 ppm; b) 50 ppm; c) 10 ppm; d) reference spectrum. Copyright Simona Cavalu et al.
HPLC profile of individual glucosinolates in broccoli sprouts (9 days old) grown under different concentrations of NSePs (10, 50 and 100 ppm). 1. PRO- Progoitrin, 2. GIB-Glucoiberin, 3. GRA- Glucoraphanin, 4. 4OHGBS- 4-hydroxyglucobrassicin, 5. GER- glucoerucin, 6. GBS-Glucobrassicin,7. MeGBS- methoxyglucobrassicin, 8. NGBS-Neoglucobrassicin. Copyright Simona Cavalu et al.
Mapping nano-Se particles in situ. Enhanced Darkfield Hyperspectral images of Se uptaken: a) 60x image of Se in broccoli leaf; b) 60x image of the leaf with Se mapped and pseudo-colored in red; c) Spectral comparison of plant tissue and Se in broccoli leaf (green line-plant tissue; red line-selenium). Copyright Simona Cavalu et al.

Growth parameters, assimilator pigments content, total phenols content and antioxidant capacity of broccoli sprouts were evaluated. The growth of seedlings was depent on NSe concentration.The treatment with 10 and 50 ppm NSe caused a slight increase in total biomass, by contrast with 100 ppm treatment. A significantly increase in amount of chlorophyll a was recorded in the case of broccoli sprouts leaves treated with 100 ppm. The content in clorophyll band total carotene was not affected by the treatment with nanoSe particles. The concentration of 100 ppm NSe particles was reflected in the highest antioxidant capacity. Our results demonstrated that NSe particles in concentration of 10, 50 and 100 ppm didn’t induce any toxicity on broccoli sprouts. The effective uptake of NSe was further demonstrated by ATR-FTIR spectroscopy and Enhanced Darkfield Hyperspectral Microscopy coupledwith CytoViva® unit. However, the complete understanding of NSe metabolism in vegetables sources requires more detailed biochemical studies and selenium uptake analysis to be conducted, especially from quantitative point of view. Copyright Simona Cavalu et al.

Full text at https://www.notulaebotanicae.ro/index.php/nbha/article/view/11490/8807